Isotope Ratio Outlier Analysis (IROA) for HPLC–TOFMS-Based Metabolomics of Human Urine
Fadi Fadil; Claudia Samol; Raffaela S. Berger; Fabian Kellermeier; Wolfram Gronwald; Peter J. Oefner; Katja Dettmer · 2022 · Metabolites
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Abstract
Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the 13 C labeled yeast extract of the IROA TruQuant kit was added as an internal standard (IS) to human urine samples measured in full-scan mode on a high-performance liquid chromatography-time-of-flight mass spectrometer (HPLC–TOFMS) system. The isotope ratio approach enabled the analysis of 112 metabolites. The correlation with reference data did not improve significan
Abstract by Fadi Fadil; Claudia Samol; Raffaela S. Berger; Fabian Kellermeier; Wolfram Gronwald; Peter J. Oefner; Katja Dettmer, Metabolites (2022) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.3390/metabo12080741