Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
Philip El-Duah; Benjamin Meyer; Augustina Sylverken; Michael Owusu; Lina Theresa Gottula; Richmond Yeboah; Jones Lamptey; Yaw Oppong Frimpong · 2019 · Viruses
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of
Abstract by Philip El-Duah; Benjamin Meyer; Augustina Sylverken; Michael Owusu; Lina Theresa Gottula; Richmond Yeboah; Jones Lamptey; Yaw Oppong Frimpong, Viruses (2019) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.3390/v11010043