Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures
Yu-Sin Jang; Hyeon Jeong Seong; Seong Woo Kwon; Yong-Suk Lee; Jung Ae Im; Haeng Lim Lee; Ye Rin Yoon; Sang Yup Lee · 2021 · Frontiers in Bioengineering and Biotechnology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the atpG gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth comp
Abstract by Yu-Sin Jang; Hyeon Jeong Seong; Seong Woo Kwon; Yong-Suk Lee; Jung Ae Im; Haeng Lim Lee; Ye Rin Yoon; Sang Yup Lee, Frontiers in Bioengineering and Biotechnology (2021) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.3389/fbioe.2021.754250