Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide
Philipp Stiefel; Sabrina Schmidt-Emrich; Katharina Maniura‐Weber; Qun Ren · 2015 · BMC Microbiology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
BACKGROUND: Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this staining method using Staphylococcus aureus and Pseudomonas aeruginosa as the model microorganisms for high throughput studies in microtiter plates. SYTO9 or PI was added alone or consecutively together to cells and the fluorescence intensities were measured using microplate reader and confocal laser sc
Abstract by Philipp Stiefel; Sabrina Schmidt-Emrich; Katharina Maniura‐Weber; Qun Ren, BMC Microbiology (2015) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1186/s12866-015-0376-x