Addition of bone morphogenetic protein type 2 to ascorbate and -glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells
Ariadne Cristiane Cabral Cruz; Mariana Lúcia Silva; Thiago Caon; Cláudia Maria Oliveira Simões · 2012 · Journal of Applied Oral Science
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
Bone morphogenetic protein type 2 (BMP-2) is a potent local factor, which promotes bone formation and has been used as an osteogenic supplement for mesenchymal stem cells. OBJECTIVES: This study evaluated the effect of a recombinant BMP-2 as well as the endogenous BMP-4 and BMP-7 in the osteogenic differentiation of adipose-derived stem cells (ASCs) in medium supplemented with ascorbate and -glycerophosphate. MATERIAL AND METHODS: Human ASCs were treated with osteogenic medium in the presence (ASCs+OM+BMP-2) or absence (ASCs+OM) of BMP-2. The alkaline phosphatase (ALP) activity was determined
Abstract by Ariadne Cristiane Cabral Cruz; Mariana Lúcia Silva; Thiago Caon; Cláudia Maria Oliveira Simões, Journal of Applied Oral Science (2012) — licensed CC BY 4.0.
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