CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs
Tim Schneider; Lee-Hsueh Hung; Silke Schreiner; Stefan Starke; Heinrich Eckhof; Oliver Roßbach; Stefan Reich; Jan Medenbach · 2016 · Scientific Reports
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
The finding, in one line
“By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells.”
Abstract
Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding pr
Abstract by Tim Schneider; Lee-Hsueh Hung; Silke Schreiner; Stefan Starke; Heinrich Eckhof; Oliver Roßbach; Stefan Reich; Jan Medenbach, Scientific Reports (2016) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1038/srep31313