Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome
Eamonn P. Culligan; Julian R. Marchesi; Colin Hill; Roy D. Sleator · 2014 · Frontiers in Microbiology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
In the current study, a number of salt-tolerant clones previously isolated from a human gut metagenomic library were screened using Phenotype MicroArray (PM) technology to assess their functional capacity. PM's can be used to study gene function, pathogenicity, metabolic capacity and identify drug targets using a series of specialized microtitre plate assays, where each well of the microtitre plate contains a different set of conditions and tests a different phenotype. Cellular respiration is monitored colorimetrically by the reduction of a tetrazolium dye. One clone, SMG 9, was found to be po
Abstract by Eamonn P. Culligan; Julian R. Marchesi; Colin Hill; Roy D. Sleator, Frontiers in Microbiology (2014) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.3389/fmicb.2014.00189