A one-step NIST traceable HPLC method for quantitation of vitamin B6 and 4-pyridoxic acid in human plasma
Xiaochun Zhang; Xiaoying Tang; Thomas M. Daly · 2020 · Practical Laboratory Medicine
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Abstract
Objectives: Vitamin B6 deficiency is associated with a wide spectrum of clinical syndromes. While vitamin B6 status is primarily assessed by measuring the biologically active form of the vitamin, pyridoxal 5-phosphate (PLP), concurrent measurement of the final metabolite 4-pyridoxic acid (PA) can provide additional information regarding supplement intake and hypophosphatasia. The aim of this study is to develop a simple method traceable to the NIST standard reference material 3950 for simultaneous detection of PLP and PA. Design & methods: A one-step reverse phase HPLC method with fluorescence
Abstract by Xiaochun Zhang; Xiaoying Tang; Thomas M. Daly, Practical Laboratory Medicine (2020) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.1016/j.plabm.2020.e00160