Mycobacterium tuberculosis Rv3406 Is a Type II Alkyl Sulfatase Capable of Sulfate Scavenging
Kimberly M. Sogi; Zev J. Gartner; Mark A. Breidenbach; Mason J. Appel; Michael W. Schelle; Carolyn R. Bertozzi · 2013 · PLoS ONE
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Abstract
The genome of Mycobacterium tuberculosis (Mtb) encodes nine putative sulfatases, none of which have a known function or substrate. Here, we characterize Mtb's single putative type II sulfatase, Rv3406, as a non-heme iron (II) and α-ketoglutarate-dependent dioxygenase that catalyzes the oxidation and subsequent cleavage of alkyl sulfate esters. Rv3406 was identified based on its homology to the alkyl sulfatase AtsK from Pseudomonas putida. Using an in vitro biochemical assay, we confirmed that Rv3406 is a sulfatase with a preference for alkyl sulfate substrates similar to those processed by Ats
Abstract by Kimberly M. Sogi; Zev J. Gartner; Mark A. Breidenbach; Mason J. Appel; Michael W. Schelle; Carolyn R. Bertozzi, PLoS ONE (2013) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1371/journal.pone.0065080