Negative / Null Result ReportOpen accessMedicine· cited by 71

Mass spectrometry analysis of adipose-derived stem cells reveals a significant effect of hypoxia on pathways regulating extracellular matrix

Simone Riis Porsborg; Allan Stensballe; Jeppe Emmersen; Cristian Pablo Pennisi; Svend Birkelund; Vladimir Zachar; Trine Fink · 2016 · Stem Cell Research & Therapy

WASTE classifies this as Negative / Null Result Report · AI classification, approximate

The study found no significant effect — useful as a negative control or null benchmark for your own design.

Abstract

BACKGROUND: Adipose-derived stem cells (ASCs) are being increasingly recognized for their potential to promote tissue regeneration and wound healing. These effects appear to be partly mediated by paracrine signaling pathways, and are enhanced during hypoxia. Mass spectrometry (MS) is a valuable tool for proteomic profiling of cultured ASCs, which may help to reveal the identity of the factors secreted by the cells under different conditions. However, serum starvation which is essentially required to obtain samples compatible with secretome analysis by MS can have a significant influence on ASC

Abstract by Simone Riis Porsborg; Allan Stensballe; Jeppe Emmersen; Cristian Pablo Pennisi; Svend Birkelund; Vladimir Zachar; Trine Fink, Stem Cell Research & Therapy (2016) — licensed CC BY 4.0.

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