Does the random nature of cell-virus interactions during in vitro infections affect TCID$_{50}$ measurements and parameter estimation by mathematical models?
Christian Quirouette; Risavarshni Thevakumaran; Kyosuke Adachi; Catherine A. A. Beauchemin · 2024 · arXiv
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract (excerpt)
Endpoint dilution (TCID50) assays cannot count the number of infectious virions (IVs), and instead are limited to counting the number of Specific INfections caused by the sample (SIN). The latter depends not only on whether virions are infectious, but also on the cells and the experimental conditions under which they interact. These interactions are random and controlled by parameters such as the rates at which IVs lose infectivity, enter cells, or fail to replicate following cell entry. Here, stochastic TCID50 assays are simulated to determine how the random number of infected wells relates t
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Metadata source: arXiv