Reality check: testing the <i>in silico</i> predictions of false negative results due to mutations in SARS-CoV-2 PCR assays using templates with mismatches <i>in vitro</i>.
Otwell T; Knight B; Coryell M; Stone J; Davis P; Necciai B; Carlson P; Sozhamannan S · 2025 · Frontiers in cellular and infection microbiology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract (excerpt)
Molecular diagnostic assays are critical tools to test, diagnose and treat infectious and other diseases. For example, PCR test results have been extremely valuable during the COVID-19 pandemic, not only to provide appropriate health care…
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Metadata source: Europe PMC · DOI 10.3389/fcimb.2025.1524025