Ptbp1 is not required for retinal neurogenesis and cell fate specification
Haley Appel; Rogger P Carmen-Orozco; Clayton P Santiago; Thanh Hoang; Seth Blackshaw · 2025 · eLife
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
The finding, in one line
“While prior studies using RNA interference suggested that Ptbp1 loss promotes neurogenesis, recent genetic studies have failed to replicate glia-to-neuron conversion following Ptbp1 loss of function.”
Abstract
The RNA-binding protein Ptbp1 has been proposed as a master regulator of neuronal fate, repressing neurogenesis through its effects on alternative splicing and miRNA maturation. While prior studies using RNA interference suggested that Ptbp1 loss promotes neurogenesis, recent genetic studies have failed to replicate glia-to-neuron conversion following Ptbp1 loss of function. To evaluate the role of Ptbp1 in developmental neurogenesis in vivo, we conditionally disrupted Ptbp1 in mouse retinal progenitors. Ptbp1 was robustly expressed in both retinal progenitors and Müller glia but absent from p
Abstract by Haley Appel; Rogger P Carmen-Orozco; Clayton P Santiago; Thanh Hoang; Seth Blackshaw, eLife (2025) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.7554/elife.108331