Dynamics of tropomyosin in muscle fibers as monitored by saturation transfer EPR of bi-functional probe.
Roni F Rayes; Tamás Kálai; Kálmán Hideg; Michael A Geeves; Piotr G Fajer · 2011 · PLoS ONE
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
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Abstract
The dynamics of four regions of tropomyosin was assessed using saturation transfer electron paramagnetic resonance in the muscle fiber. In order to fully immobilize the spin probe on the surface of tropomyosin, a bi-functional spin label was attached to i,i+4 positions via cysteine mutagenesis. The dynamics of bi-functionally labeled tropomyosin mutants decreased by three orders of magnitude when reconstituted into "ghost muscle fibers". The rates of motion varied along the length of tropomyosin with the C-terminus position 268/272 being one order of magnitude slower then N-terminal domain or
Abstract by Roni F Rayes; Tamás Kálai; Kálmán Hideg; Michael A Geeves; Piotr G Fajer, PLoS ONE (2011) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.1371/journal.pone.0021277