Evaluation of quantitative polymerase chain reaction for detecting BRCA1 or BRCA2 copy number loss in high-grade serous ovarian cancer
A. J. Oswald; R. L. Hollis; M. Churchman; I. Croy; A. Ewing; J. P. Thomson; L. J. Stillie; E. Merry · 2026 · Scientific Reports
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Abstract
Abstract Copy number loss (CNL) of tumour suppressors genes, such as BRCA1/2, are frequent but under-researched tumour driver events. Accurate identification of BRCA1/2 CNL has potential therapeutic implications, particularly in high grade serous tubo-ovarian carcinoma (HGSOC), and cost-effective detection methods are desirable. This study assessed quantitative PCR (qPCR) as a method of BRCA1/2 CNL detection in HGSOC, in two patient cohorts (cohort 1 n = 355, cohort 2 n = 86) and a cell line panel (n = 10) using sequencing-based copy number (CN) assessment as a comparator (panel-based sequenci
Abstract by A. J. Oswald; R. L. Hollis; M. Churchman; I. Croy; A. Ewing; J. P. Thomson; L. J. Stillie; E. Merry, Scientific Reports (2026) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.1038/s41598-025-34516-z