Rescue of Infectious Rotavirus Reassortants by a Reverse Genetics System Is Restricted by the Receptor-Binding Region of VP4
Alexander Falkenhagen; Marno Huyzers; Alberdina A. van Dijk; Reimar Johne · 2021 · Viruses
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
The rotavirus species A (RVA) capsid contains the spike protein VP4, which interacts with VP6 and VP7 and is involved in cellular receptor binding. The capsid encloses the genome consisting of eleven dsRNA segments. Reassortment events can result in novel strains with changed properties. Using a plasmid-based reverse genetics system based on simian RVA strain SA11, we previously showed that the rescue of viable reassortants containing a heterologous VP4-encoding genome segment was strain-dependent. In order to unravel the reasons for the reassortment restrictions, we designed here a series of
Abstract by Alexander Falkenhagen; Marno Huyzers; Alberdina A. van Dijk; Reimar Johne, Viruses (2021) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.3390/v13030363