Quantitative, Time-Resolved Proteomic Analysis by Combining Bioorthogonal Noncanonical Amino Acid Tagging and Pulsed Stable Isotope Labeling by Amino Acids in Cell Culture
John D. Bagert; Yushu Joy Xie; Michael J. Sweredoski; Yutao Qi; Sonja Hess; Erin M. Schuman; David A. Tirrell · 2014 · Molecular & Cellular Proteomics
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Abstract
An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales of minutes. The method is capable of quantifying 1400 proteins produced by HeLa cells during a 30 min interval, a time scale that is inaccessible to isotope labeling techniques alone. Potential artifacts in protein quantification can be reduced to insignificant levels by limiting the extent of noncanonical amino acid t
Abstract by John D. Bagert; Yushu Joy Xie; Michael J. Sweredoski; Yutao Qi; Sonja Hess; Erin M. Schuman; David A. Tirrell, Molecular & Cellular Proteomics (2014) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1074/mcp.m113.031914